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Glycan Profiling

Glykos provides high through-put analysis of both N-linked and O-linked glycans present on antibodies and other glycoproteins. Our proprietary technology combines a fully automated sample processing system with high throughput MALDI-TOF mass spectrometry to generate high-quality and reproducible glycan profiles. The resulting qualitative and quantitative information is analyzed with our proprietary bioinformatics tools to gain the desired information related to structure-function relationships, batch-to-batch variability, process development, formulation stability, product characterization and other factors.

Glykos’ services provide a new solution to accelerate your biotherapeutic development programs by enabling rapid glycoform analysis of your samples. Our service relies on 96-well format sample processing to maximize throughput and repeatability and mass spectroscopic analysis to resolve glycan species and facilitate accurate identification of monosaccharide compositions and glycan modifications, such as O-acetylation, sulfation, and phosphorylation.

Glykos offers several service options depending on your glycan analysis needs.

Glycan profiling:

  • Optimized for screening glycans in large sample batches (e.g. during cell line selection, upstream and downstream process development, quality control, and biomarker discovery)
  • Compatible with monoclonal antibodies and other glycoproteins
  • Applicable to both N- and O-glycoproteins as well as glycosphingolipids
  • Reliable analyses from complex biological matrices such as cells and tissues
  • Analysis method: MALDI-TOF mass spectroscopy
  • Capable of detecting low abundance glycans (< 0.5% of total glycans)
  • Detailed report with glycan composition, relative proportion of total glycans and classification into biosynthetic pathways for detected glycans

Glycan structural characterization:

  • Structural analysis of glycan structures of interest with modern analytical methods including mass spectrometric fragmentation, glycosidase sequencing and NMR spectroscopy

In addition to these service options, Glykos provides glycan analyses customized to your needs utilizing our experienced staff, well-equipped laboratories and a full suite of glycan analysis techniques.

You can find pricing information here.

For further details please contact us on info@glykos.fi.