home technology partnering analytical services contacts

Pricing

1) MALDI-TOF MS based glycoprofiling

A) Determination of N-linked glycan profile

  • PNGase digestion of the proteins to liberate N-glycans
  • Permethylation of N-glycans
  • Analysis of native and permethylated glycans by MALDI-TOF MS
  • Confirmation of N-glycan structures by MS/MS fragmentation if required
  • Classification of detected glycans into biochemical pathways
  • Search for potentially immunogenic glycan structures
  • Comparison of N-glycan profiles between batches
  • Amount of protein required per sample: 100 µg
  • Pricing: For 1 sample 2000 € per sample, for 2-3 samples 1200€ per sample, for 4-10 samples 700€ per sample, and for 11-50 samples 500€ per sample. Please contact us on info@glykos.fi in case of larger sample sets.

B) Determination of O-linked glycan profile

  • Isolation of O-glycopeptides after proteolytic digest of the proteinLiberation of O-glycans by reductive β-elimination
  • Permethylation of O-glycans
  • Analysis of permethylated glycans by MALDI-TOF MS
  • Confirmation of O-glycan structures by MS/MS fragmentation if required
  • Establishment and interpretation of a general profile of O-glycans
  • Comparison of O-glycosylation profiles between batches
  • Amount of protein required per sample: 500 µg
  • Pricing: For 1 sample 2200 € per sample, for 2-3 samples 1400€ per sample, for 4-10 samples 900€ per sample and for 11-50 samples 800 € per sample. Please contact us on info@glykos.fi in case of larger sample sets.

2) Determination of N- and O-glycosylation site occupation of antibodies

  • Digestion of antibody with specific enzyme to release Fc domain and Fab fragments
  • Isolation of glycosylated fragments
  • Analysis of the fragments by MALDI-TOF MS to confirm N-glycosylation site occupancy
  • Amount of protein required per sample: 50 µg
  • Pricing: For 1-10 samples 700 € per sample and for 11-50 samples 500 € per sample. Please contact us on info@glykos.fi in case of larger sample sets.

3) Determination of N-glycosylation site occupation of glycoproteins

  • Digestion of proteins with trypsin or other appropriate proteases
  • Separation of N-glycopeptides, O-glycopeptides and peptides on specific columns
  • Deglycosylation studies
  • Peptide mapping by MALDI-TOF MS , ESI-MS or MS/MS to determine which theoretical glycosylation sites are occupied
  • Determination of N-glycan and O-glycan profiles per site based on glycopeptide mass determination
  • Comparison of site specific glycosylation between selected batches
  • Amount of protein required per sample: 500 µg
  • Pricing and amount of protein required: Depending on complexity of the protein and its glycosylation. Please contact us on info@glykos.fi for a quote.

4) Structural characterization of glycans

  • Structural characterization of glycan structure(s) of interest with a combination of methods, selected from:
    • MALDI-TOF MS/MS mass spectrometry
    • glycosidase sequencing of glycans
    • NMR spectroscopy
    • linkage pattern by periodate oxidation analysis
    • chromatography
    • chemical degradation
    • Please contact us on info@glykos.fi for a quote.

5) Analysis of glycosphingolipid glycans

  • Isolation of glycolipid fraction from the sample
  • Endoglycoceramidase liberation of glycosphingolipid glycans
  • Permethylation of glycans
  • Analysis of native and permethylated glycans by MALDI-TOF MS
    • Confirmation of glycan structures by MS/MS fragmentation if required
  • Classification of detected glycans into biochemical pathways
  • Please contact us on info@glykos.fi for a quote.

6) Analysis of dolichol lipid glycans

  • Isolation of glycolipid fraction from the sample
  • Chemical liberation of dolichol glycans
  • Permethylation of glycans
  • Analysis of native and permethylated glycans by MALDI-TOF MS
  • Confirmation of glycan structures by MS/MS fragmentation if required
  • Please contact us on info@glykos.fi for a quote.

7) Cell and tissue glycoprofiling

  • Isolation of glycoprotein and/or glycolipid fractions from the sample
  • Enzymatic and/or chemical liberation of N-, O- and/or glycosphingolipid glycans
  • Permethylation of glycans
  • Analysis of native and permethylated glycans by MALDI-TOF MS
  • Confirmation of glycan structures by MS/MS fragmentation if required
  • Classification of detected glycans into biochemical pathways
  • Search for potential biomarker glycans by comparison of N-glycan profiles between batches
  • Please contact us on info@glykos.fi for a quote.

8) Peptide mapping of proteins

  • The protein will be denatured and reduced (or left non-reduced), digested into peptides and subjected to MALDI-TOF MS peptide mapping
  • Analysis of detected peptides against sequence databases
  • Amount of protein required: 200 µg
  • Pricing: For 1-10 samples 700 € per sample and for 11-50 samples 500 € per sample. Please contact us on info@glykos.fi in case of larger sample sets.

9) Determination of intact mass of proteins

  • The protein will be analyzed by MALDI-TOF MS to evaluate the overall mass of the protein
  • Amount of protein required per sample: 50 µg
  • For 1-10 samples 700 € per sample and for 11-50 samples 500 € per sample. Please contact us on info@glykos.fi in case of larger sample sets.

Payment terms: The invoice will be issues simultaneously with the delivery of the data. In exceptionally large orders we may require partial upfront payment. Payment term is 30 days.